Approved Strains of Colletotrichum species

Several researchers published papers on re-classification of the genus Colletotrichum based on molecular phylogenetic studies during this century. They clarified that many conventional species of the genus defined by morphology consisted of plural phylogenetic species, with the establishment of new species and combinations. Many strains of the genus preserved in the NARO Genebank were re-identified based on partial sequences of so called barcode genes such as rDNA-ITS, β-tubulin-2 and Histone H3, and some of them with typical characters to each species were selected as the approved strains. Pending species and groups of the genus Colletotrichum are also examined and re-classified at present. More approved strains will be added according to the latest information.

• Reference Sato T., Moriwaki J. (2013). Molecular re-identification of strains in NIAS Genebank belonging to phylogenetic groups A2 and A4 of the Colletotrichum acutatum species complex. Microbiol. Cult. Coll. 29(1): 13-23. [jsmrs.jp]
• Reference Sato T., Moriwaki J., Misawa T. (2013). Molecular re-identification of strains of the Colletotrichum acutatum species complex deposited in the NIAS Genebank and morphological characteristics of its member species. JARQ 47(3): 295-305. [10.6090/jarq.47.295]

• *1 Species complex:
  (CASC): C. acutatum, (CBSC): C. boninense, (CDSC): C. dematium, (CGSC): C. gloeosporioides, (CMSC): C. graminicola, (COSC): C. orbiculare, (CSSC): C. spaethanum, (CTSC): C. truncatum, (CVSC): C. destructivum
• *2 Primers are as follows:
  Actin: ATGTGCAAGGCCGGTTTCGC / TACGAGTCCTTCTGGCCCAT
  CAL: GAATTCAAGGAGGCCTTCTC / CTTCTGCATCATGAGCTGGAC
  CHS-1: TGGGGCAAGGATGCTTGGAAGAAG / TGGAAGAACCATCTGTGAGAGTTG
  EF1-α: TGCGTCATCGGCCACGT / GGATGTACCAGTSATCATGTT
  GPDH: GCCGTCAACGACCCCTTCATTGA / GGGTGGAGTCGTACTTGAGCATGT
  GS: GCCGGT GGAGGAACCGTCG / GAACCGTCGAAGTTCCAC
  H3: AGGTCCACTGGTGGCAAG / AGCTGGATGTCCTTGGACTG
  HMG: ATTCTCTACCGCAAGGATC / CAGTTTGT'TATGGCGCTC
  ITS: GGAAGTAAAAGTCGTAACAAGG / TCCTCCGCTTATTGATATGC
  SOD2: GCCCACAGTACATATTGCCTAAGC / TCATCCCGGGAGCCAGAAAACCT
  β-tubulin: AACATGCGTGAGATTGTAAGT / ACCCTCAGTGTAGTGACCCTTGGC