Culture Media
- For reviving freeze-dried cultures, the following liquid media without agar are used.
PDA ‘BD Difco’, AccuDia Corn Meal Agar ‘Shimadzu Diagnostics’ (CMA), AccuDia Standard Method Agar ‘Shimadzu Diagnostics’ (SMA) commercially sold is not applicable for preparing liquid culture, because it contains agar in its formula for solidification. - Media should be sterilized at 121°C for 15-20 minutes.
- Media without pH indication are those for which pH adjustment is unnecessary.
media for filamentous fungi and yeasts
| PDA ‘BD Difco’ | 39.0 g/l |
| Agar | 3.0 g/l |
Although PDA ‘BD Difco’ contains agar, 3.0g/l more agar could preferably be added to get a harder agar surface for better practice of microbial inoculation/transplantation
| AccuDia Corn Meal Agar ‘Shimadzu Diagnostics’ | 17.0 g/l |
| Agar | 3.0 g/l |
To prepare culture medium for marine fungi, 38.0g/l of dried seawater powder (A JAPAN PET DRUGS CO.,LTD.) could be added to it.
| Glucose | 0.2 g/l |
| Sucrose | 0.2 g/l |
| KH2PO4 | 1.0 g/l |
| KNO3 | 1.0 g/l |
| MgSO4・7H2O | 0.5 g/l |
| KCl | 0.5 g/l |
| Agar | 20.0 g/l |
| EBIOS powder | 5.0 g/l |
| Glucose | 20.0 g/l |
| Agar | 18.0 g/l |
| Yeast extract | 3.0 g/l |
| Malt extract | 3.0 g/l |
| Peptone | 5.0 g/l |
| Glucose | 10.0 g/l |
| Agar | 18.0 g/l |
media for Bacteria
| AccuDia Standard Method Agar ‘Shimadzu Diagnostics’ | 23.5 g/l |
| Agar | 3.0 g/l |
Though AccuDia Standard Method Agar ‘Shimadzu Diagnostics’ contains agar, we add 3.0g/l more agar to it in order to stiffen it and inoculate easier.
| Yeast extract | 0.4 g/l |
| Mannitol | 10.0 g/l |
| K2HPO4 | 0.5 g/l |
| MgSO4・7H2O | 0.2 g/l |
| NaCl | 0.1 g/l |
| Agar | 18.0 g/l |
| Peptone | 10.0 g/l |
| Casein hydrolysate | 1.0 g/l |
| Glucose | 5.0 g/l |
| 1% solution of TTC | 5 ml/l |
| Agar | 18.0 g/l |
| MRS medium ‘BD Difco’ | 52.0 g/l |
| Agar | 18.0 g/l |
media for Actinomyces
| Yeast extract | 4.0 g/l |
| Malt extract | 10.0 g/l |
| Glucose | 4.0 g/l |
| Agar | 18.0 g/l |
| pH 7.3 |
| Yeast extract | 2.0 g/l |
| soluble starch | 15.0 g/l |
| K2HPO4 | 0.5 g/l |
| MgSO4・7H2O | 0.5 g/l |
| Agar | 18.0 g/l |
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