Culture Media
- For reviving freeze-dried cultures, the following liquid media without agar are used.
PDA ‘BD Difco’, Corn meal extract powder ‘Nissui’ (CMA), Standard Methods Agar ‘Nissui’ (SMA) commercially sold is not applicable for preparing liquid culture, because it contains agar in its formula for solidification. - Media should be sterilized at 121°C for 15-20 minutes.
- Media without pH indication are those for which pH adjustment is unnecessary.
media for filamentous fungi and yeasts
PDA ‘BD Difco’ | 39.0 g/l |
Agar | 3.0 g/l |
Although PDA ‘BD Difco’ contains agar, 3.0g/l more agar could preferably be added to get a harder agar surface for better practice of microbial inoculation/transplantation
Corn meal extract powder ‘Nissui’ | 17.0 g/l |
Agar | 3.0 g/l |
To prepare culture medium for marine fungi, 38.0g/l of dried seawater powder (A JAPAN PET DRUGS CO.,LTD.) could be added to it.
- Mix 400 mL of commercially available V8 juice with 600 mL of distilled water and 4.5 g of CaCO3. After stirring, centrifuge the mixture.
- Add 800 mL of distilled water and 18.0 g of agar to 200 mL of the obtained supernatant.
Glucose | 0.2 g/l |
Sucrose | 0.2 g/l |
KH2PO4 | 1.0 g/l |
KNO3 | 1.0 g/l |
MgSO4・7H2O | 0.5 g/l |
KCl | 0.5 g/l |
Agar | 20.0 g/l |
EBIOS powder | 5.0 g/l |
Glucose | 20.0 g/l |
Agar | 18.0 g/l |
Yeast extract | 3.0 g/l |
Malt extract | 3.0 g/l |
Peptone | 5.0 g/l |
Glucose | 10.0 g/l |
Agar | 18.0 g/l |
media for Bacteria
Standard Methods Agar ‘Nissui’ | 23.5 g/l |
Agar | 3.0 g/l |
Though Standard Methods Agar ‘Nissui’ contains agar, we add 3.0g/l more agar to it in order to stiffen it and inoculate easier.
Yeast extract | 0.4 g/l |
Mannitol | 10.0 g/l |
K2HPO4 | 0.5 g/l |
MgSO4・7H2O | 0.2 g/l |
NaCl | 0.1 g/l |
Agar | 18.0 g/l |
Peptone | 10.0 g/l |
Casein hydrolysate | 1.0 g/l |
Glucose | 5.0 g/l |
1% solution of TTC | 5 ml/l |
Agar | 18.0 g/l |
MRS medium ‘BD Difco’ | 52.0 g/l |
Agar | 18.0 g/l |
media for Actinomyces
Yeast extract | 4.0 g/l |
Malt extract | 10.0 g/l |
Glucose | 4.0 g/l |
Agar | 18.0 g/l |
pH 7.3 |
Yeast extract | 2.0 g/l |
soluble starch | 15.0 g/l |
K2HPO4 | 0.5 g/l |
MgSO4・7H2O | 0.5 g/l |
Agar | 18.0 g/l |
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