Culture Media

Culture medium
  • For reviving freeze-dried cultures, the following liquid media without agar are used.
    PDA ‘BD Difco’, Corn meal extract powder ‘Nissui’ (CMA), Standard Methods Agar ‘Nissui’ (SMA) commercially sold is not applicable for preparing liquid culture, because it contains agar in its formula for solidification.
  • Media should be sterilized at 121°C for 15-20 minutes.
  • Media without pH indication are those for which pH adjustment is unnecessary.

media for filamentous fungi and yeasts

medium composition
PDA ‘BD Difco’ 39.0 g/l
Agar 3.0 g/l
applicable for most of filamentous fungi
Although PDA ‘BD Difco’ contains agar, 3.0g/l more agar could preferably be added to get a harder agar surface for better practice of microbial inoculation/transplantation

medium composition
Corn meal extract powder ‘Nissui’ 17.0 g/l
Agar 3.0 g/l
Although Corn meal extract powder ‘Nissui’ contains agar, 3.0g/l more agar could preferably be added to get a harder agar surface for better practice of microbial inoculation/transplantation
To prepare culture medium for marine fungi, 38.0g/l of dried seawater powder (A JAPAN PET DRUGS CO.,LTD.) could be added to it.

  1. Mix 400 mL of commercially available V8 juice with 600 mL of distilled water and 4.5 g of CaCO3. After stirring, centrifuge the mixture.
  2. Add 800 mL of distilled water and 18.0 g of agar to 200 mL of the obtained supernatant.
medium for Oomycetes.

medium composition
Glucose 0.2 g/l
Sucrose 0.2 g/l
KH2PO4 1.0 g/l
KNO3 1.0 g/l
MgSO4・7H2O 0.5 g/l
KCl 0.5 g/l
Agar 20.0 g/l
medium for classification and identification of Nectria group (Fusarium, Cylindrocladium, Cylindrocarpon, Acremonium)

medium composition
EBIOS powder 5.0 g/l
Glucose 20.0 g/l
Agar 18.0 g/l
medium for Basidomycetes.

medium composition
Yeast extract 3.0 g/l
Malt extract 3.0 g/l
Peptone 5.0 g/l
Glucose 10.0 g/l
Agar 18.0 g/l
medium for Yeasts.

media for Bacteria

medium composition
Standard Methods Agar ‘Nissui’ 23.5 g/l
Agar 3.0 g/l
medium for most bacteria.
Though Standard Methods Agar ‘Nissui’ contains agar, we add 3.0g/l more agar to it in order to stiffen it and inoculate easier.

medium composition
Yeast extract 0.4 g/l
Mannitol 10.0 g/l
K2HPO4 0.5 g/l
MgSO4・7H2O 0.2 g/l
NaCl 0.1 g/l
Agar 18.0 g/l
medium for Bradyrhizobium, Mesorhizobium, Rhizobium, Sinorhizobium (Ensifer).

medium composition
Peptone 10.0 g/l
Casein hydrolysate 1.0 g/l
Glucose 5.0 g/l
1% solution of TTC 5 ml/l
Agar 18.0 g/l
medium for Ralstonia solanacearum, R. pseudosolanacearum, R. syzygii. TTC solution (filtered through a 0.2µ filter) should be added at 50-60℃ after autoclaving a mixture of other components.

medium composition
MRS medium ‘BD Difco’ 52.0 g/l
Agar 18.0 g/l
for lactic acid bacteria.

media for Actinomyces

medium composition
Yeast extract 4.0 g/l
Malt extract 10.0 g/l
Glucose 4.0 g/l
Agar 18.0 g/l
pH 7.3
for most Actinomycetes.

medium composition
Yeast extract 2.0 g/l
soluble starch 15.0 g/l
K2HPO4 0.5 g/l
MgSO4・7H2O 0.5 g/l
Agar 18.0 g/l
medium for growth of genus Streptomyces.