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Culture Media
- For reviving freeze-dried cultures, the following liquid media without agar are used.
PDA ‘BD Difco’, Corn meal extract powder ‘Nissui’ (CMA), Standard Methods Agar ‘Nissui’ (SMA), King B Agar ‘Eiken’ medium commercially sold is not applicable for preparing liquid culture, because it contains agar in its formula for solidification. - Media should be sterilized at 121°C for 15-20 minutes.
- Media without pH indication are those for which pH adjustment is unnecessary.
media for filamentous fungi and yeasts
PDA (Potato Dextrose Agar)
PDA ‘BD Difco’ | 39.0 g/l |
Agar | 3.0 g/l |
Although PDA ‘BD Difco’ contains agar, 3.0g/l more agar could preferably be added to get a harder agar surface for better practice of microbial inoculation/transplantation
CMA (Corn Meal Agar)
Corn meal extract powder ‘Nissui’ | 17.0 g/l |
Agar | 3.0 g/l |
To prepare culture medium for marine fungi, 38.0g/l of dried seawater powder (A JAPAN PET DRUGS CO.,LTD.) could be added to it.
V-8 medium
- Mix 400 mL of commercially available V8 juice with 600 mL of distilled water and 4.5 g of CaCO3. After stirring, centrifuge the mixture.
- Add 800 mL of distilled water and 18.0 g of agar to 200 mL of the obtained supernatant.
SNA (Synthetic low-Nutrient Agar)
Glucose | 0.2 g/l |
Sucrose | 0.2 g/l |
KH2PO4 | 1.0 g/l |
KNO3 | 1.0 g/l |
MgSO4・7H2O | 0.5 g/l |
KCl | 0.5 g/l |
1N NaOH | 0.6 ml/l |
Agar | 23.0 g/l |
LCA (Miura medium)
Yeast extract | 0.2 g/l |
Glucose | 1.0 g/l |
NaNO3 | 2.0 g/l |
KH2PO4 | 1.0 g/l |
KCl | 0.2 g/l |
MgSO4・7H2O | 0.2 g/l |
Agar | 18.0 g/l |
pH 6.5-7.0 |
IFO-7 medium
EBIOS powder | 5.0 g/l |
Glucose | 20.0 g/l |
Agar | 18.0 g/l |
IFO-9 medium
Sucrose | 200.0 g/l |
NaNO3 | 3.0 g/l |
K2HPO4 | 1.0 g/l |
MgSO4・7H2O | 0.5 g/l |
KCl | 0.5 g/l |
FeSO4・7H2O | 0.01 g/l |
Agar | 18.0 g/l |
pH 6.0 |
YM (Yeast Malt peptone agar)
Yeast extract | 3.0 g/l |
Malt extract | 3.0 g/l |
Peptone | 5.0 g/l |
Glucose | 10.0 g/l |
Agar | 18.0 g/l |
pH 7.0 |
media for Bacteria
YPGS(Yeast extract Peptone Glucose Saccharose)
Yeast extract | 1.0 g/l |
Peptone | 10.0 g/l |
Glucose | 5.0 g/l |
Sucrose | 5.0 g/l |
NaCl | 3.0 g/l |
pH 7.0 |
SMA (Standard Methods Agar)
Standard Methods Agar ‘Nissui’ | 23.5 g/l |
Agar | 3.0 g/l |
Though Standard Methods Agar ‘Nissui’ contains agar, we add 3.0g/l more agar to it in order to stiffen it and inoculate easier.
medium for Bradyrhizobium
Yeast extract | 0.4 g/l |
Mannitol | 10.0 g/l |
K2HPO4 | 0.5 g/l |
MgSO4・7H2O | 0.2 g/l |
NaCl | 0.1 g/l |
Agar | 18.0 g/l |
pH 6.8 |
TTC(2,3,5-Triphenyl Tetrazolium Chloride)
Peptone | 10.0 g/l |
Casein hydrolysate | 1.0 g/l |
Glucose | 5.0 g/l |
1% solution of TTC | 1ml g/l |
Agar | 15.0 g/l |
MRS (de Man-Rogosa-Sharpe)
MRS medium ‘OXOID’ | 52.0 g/l |
Agar | 18.0 g/l |
King B
King B Agar ‘Eiken’ | 38.0 g/l |
Glycerin | 10.0 g/l |
YMA (Yeast Malt extract Agar)
YMA(Yeast extract-Malt extract Agar)
Yeast extract | 4.0 g/l |
Malt extract | 10.0 g/l |
Glucose | 4.0 g/l |
Agar | 18.0 g/l |
pH 7.3 |
Albumin medium
Albumin medium | 0.25 g/l |
Glucose | 1.0 g/l |
K2HPO4 | 0.5 g/l |
MgSO4・7H2O | 0.2 g/l |
1% solution of Fe2(SO4)3 | 1 ml |
Agar | 18.0 g/l |
pH 6.8-7.0 |